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Characterization of protoberberine alkaloids in C optidis R hizoma ( H uanglian) by HPLC with ESI ‐ MS / MS
Author(s) -
Ren Lingling,
Xue Xingya,
Liang Xinmiao
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201201002
Subject(s) - chemistry , high performance liquid chromatography , chromatography , tandem mass spectrometry , alkaloid , mass spectrometry , stereochemistry
This study aims to qualitatively analyze protoberberine alkaloids in crude extract of C optidis R hizoma using HPLC with ESI ‐ MS / MS . Possible specific molecular weights of protoberberine alkaloids were firstly deduced according to literatures and were adopted to screen the alkaloids in the HPLC with ESI ‐ MS of crude extract of C optidis R hizoma. As a result, 21 protoberberine alkaloids were found, including compounds of very low concentration and compounds coeluted in one peak. Among these, two compounds were positively identified and verified by comparison with standards. Ten of these compounds were first reported in this study for C optidis R hizoma. In addition, chromatographic retention parameters a and c of all compounds were obtained using their retention times under five gradient conditions and were applied to confirm the deduction about the structures of protoberberine alkaloids by tandem mass data.