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Separation and pre‐concentration of glucocorticoids in water samples by ionic liquid supported vortex‐assisted synergic microextraction and HPLC determination
Author(s) -
Qin Hui,
Li Bi,
Liu Mou Sheng,
Yang Ya Ling
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200989
Subject(s) - chromatography , chemistry , extraction (chemistry) , ionic liquid , high performance liquid chromatography , tap water , ionic strength , detection limit , aqueous solution , organic chemistry , environmental engineering , engineering , catalysis
We have developed a synergic microextraction procedure based on ionic liquid for the pre‐concentration and determination of glucocorticoids in water samples. Using nonionic surfactant T riton X ‐100 ( TX ‐100) as synergic reagent, 1‐butyl‐3‐methylimidazolium hexa‐fluorophosphate accomplished extraction rapidly without heating in water bath. One key property of ionic liquids that highlights their potential is their wide liquid temperature range. The improved extraction was named as ionic liquid supported vortex‐assisted synergic microextraction. Compared with the traditional liquid–liquid extraction and cloud point extraction, ionic liquid supported vortex‐assisted synergic microextraction was accomplished in 8 min with considerably high recovery. The proposed method greatly improved the sensitivity of HPLC for the determination of glucocorticoids. The results obtained indicated a good linearity with the correlation coefficient of 0.997 over the range of 0.6–300 ng/mL and high sensitivity with LOD s of 4.11, 9.19, and 7.50 ng/mL for hydrocortisone butyrate, beclomethasone dipropionate, and nandrolone phenylpropionate, respectively. The RSD of the method was 1.57–1.81% ( n = 6) with enrichment factor of 99.85, and good recovery (≥97.24%). The method was successfully applied to the determination of glucocorticoids in mineral water, water of D ianchi lake, and tap water samples.