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Sensitive monitoring of penicillin antibiotics in milk and honey treated by stir bar sorptive extraction based on monolith and LC ‐electrospray MS detection
Author(s) -
Huang Xiaojia,
Chen Linli,
Chen Meng,
Yuan Dongxing,
g Shuyu
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200987
Subject(s) - chromatography , chemistry , dicloxacillin , extraction (chemistry) , cloxacillin , detection limit , penicillin , benzylpenicillin , electrospray , mass spectrometry , antibiotics , biochemistry
In the present study, a convenient and sensitive method for determination of six penicillin antibiotics (amoxicillin, ampicillin, penicillin G , oxacillin, cloxacillin, and dicloxacillin) in milk and honey samples was developed. Milk and honey samples were diluted with water, then directly treated by stir bar sorptive extraction based on poly (vinylimidazole‐divinylbenzene) monolithic material as coating. The analytes were analyzed by LC/ESI‐ MS/MS . Several extraction parameters including extraction and desorption time, p H value, and ionic strength in sample matrix were investigated in detail. Under the optimized extraction conditions, the calculated detection limits for the target compounds were as low as 0.23–2.66 ng/kg in milk and 0.18–1.42 ng/kg in honey, respectively. Good linearity was obtained for analytes with the correlation coefficients ( R 2 ) above 0.997. Excellent method reproducibility was achieved in terms of intraday and interday precisions, indicated by the RSD s of <5.0 and <10.0%, respectively. Finally, the proposed method was successfully applied to the determination of penicillin antibiotics residues in different milk and honey samples.