Premium
Pharmacokinetic study of schisandrin, schisandrol B , schisantherin A , deoxyschisandrin, and schisandrin B in rat plasma after oral administration of S hengmaisan formula by UPLC ‐ MS
Author(s) -
Sun Hui,
Wu Fangfang,
Zhang Aihua,
Wei Wenfeng,
Han Ying,
Wang Xijun
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200887
Subject(s) - pharmacokinetics , chromatography , formic acid , chemistry , high performance liquid chromatography , oral administration , calibration curve , pharmacology , medicine , detection limit
Shengmaisan (SMS) is a traditional C hinese medicine prescription widely used for the treatment of cardiovascular diseases in A sia. Its lignans are major components responsible for therapeutic action. A rapid and specific UPLC ‐ Q ‐ TOF / MS has been developed and validated for simultaneous quantification of the five main bioactive components, i.e. schisandrin, schisandrol B , schisantherin A , deoxyschisandrin, and schisandrin B , in rat plasma after oral administration of SMS . All calibration curves showed excellent linearity within the test ranges. Validation proved the repeatability of the method was good and recovery was satisfactory. The separation of these compounds was carried out on a W aters ACQUITY HSS T 3 column (2.1 × 100 mm, 1.8 μm) by linear gradient elution using a mobile phase consisting of 0.01% formic acid in water and ACN containing 0.01% formic acid. In this work, plasma pharmacokinetic characteristics of lignans components after oral administration SMS were investigated using UPLC ‐ Q ‐ TOF / MS method. MS was performed on a W aters M icromass high‐definition technology with an ESI source. Data were analyzed and estimated by compartmental methods and pharmacokinetic parameters calculated using W in N onlin Professional version 6.1. Results demonstrated that the proposed UPLC ‐ Q ‐ TOF / MS method was successfully applied to pharmacokinetic study of all components in rat plasma after oral administration of the SMS .