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Simultaneous determination of seven lignans in J usticia procumbens by high performance liquid chromatography–photodiode array detection using relative response factors
Author(s) -
Luo Zuliang,
Kong Weijun,
Qiu Feng,
Yang Meihua,
Li Qian,
Wei Riwei,
Yang Xiaoli,
Qin Jieping
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200851
Subject(s) - chromatography , high performance liquid chromatography , photodiode , lignan , chemistry , acetonitrile , elution , gradient elution , analytical chemistry (journal) , materials science , stereochemistry , optoelectronics
A simple and sensitive HPLC coupled with photodiode array ( HPLC–PDA ) method was developed for simultaneous determination of seven lignans in Justicia procumbens using relative response factors ( RRF s). The chromatographic separation was performed on a S hiseido C apcell P ak C 18 column (250 × 4.6 mm id, 5 μm), a gradient elution of acetonitrile/water, and a photodiode array detector. The column temperature was maintained at 35° C and the detection wavelength was set at 256 nm. Chinensinaphthol methyl ether was selected as the reference compound for calculating the relative response factors of the lignans. It has shown that the RRF s for lignans are quite similar at 256 nm of detection under different analytical conditions (different columns and HPLC instruments). Using RRF s, not every lignan is needed as a reference standard, making the method ideal for rapid, routine analysis, especially for those laboratories where lignans standards are not readily available. An economic and practicable HPLC method using RRF s was established for the determination of seven lignans in J . procumbens . This method not only can determine multiple indexes in traditional Chinese medicines (TCMs) simultaneously, but also resolve the problem of lacking of chemical standards. It will be a good quality evaluation method and pattern for TCMs.