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Development and evaluation of a new in vivo solid‐phase microextraction sampler
Author(s) -
Togunde Oluranti P.,
Lord Heather,
Oakes Ken D.,
Servos Mark R.,
Pawliszyn Janusz
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200839
Subject(s) - solid phase microextraction , chromatography , in vivo , detection limit , fish <actinopterygii> , reproducibility , chemistry , sampling (signal processing) , biomedical engineering , computer science , gas chromatography–mass spectrometry , mass spectrometry , biology , microbiology and biotechnology , medicine , fishery , filter (signal processing) , computer vision
The use of solid‐phase microextraction ( SPME ) as a nonlethal technique for in vivo sampling of pharmaceutical residue in fish tissue has been documented in the literature. However, there is need to improve its simplicity and robustness for wider applications in the laboratory and field. The objective of this research is to develop and improve the SPME device for sampling of pharmaceuticals in fish tissue. The practical application of the new device was demonstrated in the field where some wild fish ( E sox masquinongy ) were caught in the river and sampled by the device. The samples were analyzed using LC coupled with MS / MS ( LC ‐ MS / MS ). The new in vivo SPME device with a PDMS extraction phase (sorbent) was demonstrated to a robust tool by both experts and nonexpert of the method and it is simpler than the traditional device. The detection limit of the method in gel and fish tissue was 0.01–0.26 ng/g. The interday reproducibility in gel and fish homogenized fish tissue was 8–16% RSD . This study demonstrates that the new device will provide a platform or opportunity for rapid sampling of carbamazepine, diazepam, and nordiazepam in fish muscle with acceptable precision.