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Selectivity tuning via temperature pulsing using low thermal mass liquid chromatography and monolithic columns
Author(s) -
Pursch Matthias,
Eckerle Patric,
Gu Binghe,
Luong Jim,
Cortes Hernan J.
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200818
Subject(s) - selectivity , resolution (logic) , chromatography , chemistry , elution , capillary action , analytical chemistry (journal) , temperature gradient , thermal , high performance liquid chromatography , materials science , thermodynamics , computer science , biochemistry , physics , quantum mechanics , artificial intelligence , composite material , catalysis
Low thermal mass LC was applied to the capillary LC separation of a complex insecticide mixture by increasing temperature and decreasing gradients, as well as fast selected temperature pulses to increase resolution of overlapped components. The technology was applied using a new generation of capillary monolithic stationary phases. Considerable peak shifts and selectivity changes were observed for given temperature conditions. The concept of temperature pulsing during an elution profile shows promise for increasing resolution in difficult separations and can provide a relatively simple means to solve coelution problems.