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Development of an analytical method for yam saponins using HPLC with pulsed amperometric detection at different column temperatures
Author(s) -
Kwon HaJeong,
Choi SeungHee,
Yoo ChangSeon,
Choi HwaYoung,
Lee SooEon,
Park YongDuk
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200756
Subject(s) - saponin , chromatography , chemistry , resolution (logic) , high performance liquid chromatography , retention time , dioscorea , detection limit , amperometry , medicine , alternative medicine , pathology , artificial intelligence , computer science , electrode , electrochemistry
Yam saponins (dioscin, gracillin, protodioscin, and protogracillin) were analyzed with three different C18 columns at incremental column temperatures from 15 to 45°C to investigate the effect of temperature on the retention and resolution of yam saponins. At low temperature, yam saponins showed decreased retention times and improved resolutions in the C18 columns. In the Kinetex C18 column at 15°C, the four saponins achieved baseline separation (Rs > 1.5) within 30 min. Pulsed amperometric detection was used to identify saponins with high sensitivity. The limits of detection and quantification of saponins were 0.11–0.31 and 0.33–0.95 ng, respectively. The correlation coefficients ranged 0.9986–1.0000. Intra‐ and inter‐day precisions were <4.2% of retention times and <9.5% of the calculated contents. Average recoveries ranged from 92.18 to 105.98%. Saponin contents in Dioscorea nipponica tubers and commercial yam foods were determined without sample purification or concentration. Among the ten commercial yam foods investigated, only three showed significant saponin contents.