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Analysis of aflatoxins in nonalcoholic beer using liquid–liquid extraction and ultraperformance LC ‐ MS / MS
Author(s) -
Khan Mohammad R.,
Alothman Zeid A.,
Ghfar Ayman A.,
Wabaidur Saikh M.
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200752
Subject(s) - aflatoxin , chromatography , repeatability , extraction (chemistry) , calibration curve , chemistry , ethyl acetate , analyte , liquid chromatography–mass spectrometry , detection limit , mass spectrometry , food science
Aflatoxins AFB 1, AFB 2, AFG 1, and AFG 2 are toxic secondary metabolites produced by A spergillus flavus and A spergillus parasiticus and posses a potential threat to food safety. In the present work, liquid–liquid extraction and ultraperformance LC ‐ MS / MS method has been applied for the determination of four naturally occurring aflatoxins AFB 1, AFB 2, AFG 1, and AFG 2 in nonalcoholic beer. Aflatoxins extraction from nonalcoholic beer was carried out using liquid–liquid extraction procedure. The effects of solvent‐types were studied to obtain maximum recovery of the target analytes with minimum contamination. Among different solvents, the aflatoxins extraction was best achieved using ethyl acetate. The obtained recoveries were ranged from 85 to 96% with good quality parameters: LOD values between 0.001 and 0.003 ng/mL, linearity of the calibration curve ( r 2 > 0.999), and repeatability (run‐to‐run) and reproducibility (day‐to‐day) precisions with RSD s lower than 5% ( n = 5) achieved at 0.50 ng/mL concentration. The optimized liquid–liquid extraction in combination with ultraperformance LC ‐ MS / MS was applied successfully to the analysis of AFB 1, AFB 2, AFG 1, and AFG 2 aflatoxins in 11 nonalcoholic beers and were detected up to 15.31 ng/L in some of the samples.