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Analysis of free amino acids in A mur s turgeon by ultra‐performance liquid chromatography using pre‐column derivatization with 6‐aminoquinolyl‐carbamyl
Author(s) -
Sun Yanchun,
Xu Xianzhu,
Mou Zhenbo,
Wang Jing,
Tan Zhijun,
Wu Song
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200733
Subject(s) - chromatography , amino acid , high performance liquid chromatography , chemistry , sturgeon , derivatization , repeatability , reagent , resolution (logic) , acetonitrile , fish <actinopterygii> , biology , organic chemistry , biochemistry , artificial intelligence , fishery , computer science
A rapid, sensitive, and reliable ultra‐performance liquid chromatography ( UPLC ) coupled with photodiode array detection method was developed for the amino acid analysis of A mur s turgeon ( A cipenser schrenckii B randt). The method uses minimal sample volume and automated online precolumn derivitization of amino acids with fluorescent 6‐aminoquinolyl‐carbamyl reagent. The chromatographic separation was achieved by UPLC , which used a column with 1.7 μm particle packing that enabled higher speed of analysis, peak capacity, greater resolution, and increased sensitivity. Amino acid derivatives obtained under optimal conditions were separated on a W aters UPLC BEH C 18 column with Acetonitrile –acetate buffer as mobile phase. Matrix effects were investigated and good linearities with correlation coefficients better than 0.9949 were obtained over a wide range of 5–1000 μmol/L for all amino acids. The simple sample preparation and minimal sample volume make the method useful for the quantitation of 17 amino acids in A mur s turgeon samples. It is concluded that a rapid and robust platform based on UPLC was established, and a total of 17 amino acids of A mur s turgeon were tentatively detected. This method showed good accuracy and repeatability that can be used for the quantification of amino acids in real samples.