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Solid‐phase microextraction based method for determination of essential oils components in herbal beverages
Author(s) -
Adam Martin,
Čížková Andrea,
Eisner Aleš,
Ventura Karel
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200714
Subject(s) - solid phase microextraction , repeatability , chromatography , sorption , extraction (chemistry) , chemistry , desorption , sample preparation , flame ionization detector , gas chromatography , mass spectrometry , gas chromatography–mass spectrometry , adsorption , organic chemistry
A method employing the direct immersion solid‐phase microextraction followed by GC‐MS analysis is presented for the determination of essential oils components in herbal tea infusions, i.e. their direct content in the liquid phase. The extraction performances were compared using five different microextraction fibres. Significant parameters affecting sorption process such as sample amount, sorption and desorption time and temperature, stirring speed, pH adjustment and effect of ionic strength were optimised and discussed. By optimising the key parameters, a detection limits (LOD = S/N × 3) for ten target marker compounds were obtained in the range from 5.3 to 48.2 ng/mL with recoveries ranged between 93.03 and 100.50%. Intra‐day and inter‐day repeatability at three concentration levels were found to be 1.1–15.3 and 7.2–15.5% RSD, respectively. Finally, the optimised procedure enabling a rapid and simple analysis of essential oils was applied for the direct determination of these compounds in ten herbal tea infusions.