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Monitoring of alcohol markers by capillary electrophoresis
Author(s) -
Caslavska Jitka,
Thormann Wolfgang
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200706
Subject(s) - ethyl glucuronide , phosphatidylethanol , carbohydrate deficient transferrin , chromatography , acetaldehyde , capillary electrophoresis , alcohol , chemistry , transferrin , ethanol , biochemistry , alcohol consumption , phospholipid , membrane , phosphatidylcholine
Work dealing with the monitoring of alcohol markers by CE performed during the past two decades led to the development of assays for carbohydrate‐deficient transferrin ( CDT ), ethyl sulfate, ethyl glucuronide, and phosphatidylethanol in body fluids and first attempts for the detection of the urinary 5‐hydroxytryptophol/5‐hydroxyindoleacetic acid ratio and stable hemoglobin acetaldehyde adducts. Most notably are assays for CDT that have been commercialized and are being used in many laboratories under routine conditions. This paper provides insight into the development, specifications, and use of the currently known CE ‐based assays suitable to detect alcohol markers. The achievements reached so far indicate that CE is an attractive technology for monitoring alcohol markers. This is particularly seen with the CDT assays that do not require an elaborate sample pretreatment and thus could be fully automated for high‐throughput analyses on multicapillary instruments.