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Separation selectivity patterns of fully charged achiral compounds in capillary electrophoresis with a neutral cyclodextrin
Author(s) -
Soonthorntantikul Wasura,
Srisaart Monpichar,
Leepipatpiboon Natchanun,
Nhujak Thumnoon
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200705
Subject(s) - selectivity , chemistry , capillary electrophoresis , analyte , cyclodextrin , electrophoresis , chromatography , structural isomer , dimensionless quantity , analytical chemistry (journal) , organic chemistry , catalysis , physics , mechanics
Based on the separation selectivity equation, related to the dimensionless parameters for fully charged achiral analytes using a neutral CD, the separation selectivity can be classified into seven patterns. With respect to CZE without CD, the presence of CD in the buffer may improve, or reduce, the separation selectivity with this effect being accompanied by the same or reversed electrophoretic mobility order for charged analytes. This can depend on the separation selectivity of the two analytes in free solution, the binding selectivity, the separation selectivity of analyte–CD complexes and the ratio of electrophoretic mobility of the analytes in free, and complexed forms. Using positional isomers of benzoic acids and phenoxy acids as test analytes and α‐CD as a selector, the observed separation selectivity shapes were found to be in excellent agreement with the predicted separation selectivities.