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Simultaneous determination of quinoxaline‐1,4‐dioxides in feeds using molecularly imprinted solid‐phase extraction coupled with HPLC
Author(s) -
He Qianqian,
Fang Binghu,
Su Yijuan,
Zeng Zhenling,
Yang Jianwen,
He Limin,
Zeng Dongping
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200701
Subject(s) - molecularly imprinted polymer , solid phase extraction , chromatography , chemistry , analyte , quinoxaline , detection limit , extraction (chemistry) , high performance liquid chromatography , molecular imprinting , acrylamide , selectivity , polymer , monomer , organic chemistry , catalysis
A simple, selective, and reproducible molecularly imprinted SPE coupled with HPLC method was developed for monitoring quinoxaline‐1,4‐dioxides in feeds. Molecularly imprinted polymers were synthesized in methanol using mequindox ( MEQ ) as template molecule and acrylamide as functional monomer by bulk polymerization. Under the optimum SPE conditions, the novel polymer sorbents can selectively extract and enrich carbadox, MEQ , quinocetone, and cyadox from a variety of feeds. The molecularly imprinted SPE cartridge was better than nonimprinted, C 18 , and HLB cartridges in terms of both recovery and precision. Mean recoveries of four quinoxaline‐1,4‐dioxides from six kinds of feeds spiked at 1.0, 10, and 100 mg/kg ranged between 75.2 and 94.7% with RSD s of less than 10%. The decision limits (CCαs) and the detection capabilities (CCβs) of four analytes were 0.15–0.20 mg/kg and 0.44–0.56 mg/kg, respectively. The class selectivity of the polymers was evaluated by checking three drugs with different molecular structures to that of MEQ .