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Performance evaluation of ion‐exchange chromatography in capillary format
Author(s) -
Eghbali Hamed,
Bruggink Cees,
Agroskin Yury,
Pohl Christopher A.,
Eeltink Sebastiaan
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200680
Subject(s) - capillary action , chemistry , chromatography , analytical chemistry (journal) , ion exchange , ion chromatography , ion , capillary electrochromatography , ion exchange resin , capillary electrophoresis , materials science , composite material , inorganic chemistry , organic chemistry
The performance of a recently introduced capillary ion‐exchange chromatography system was explored. Experiments were conducted in isocratic mode with a commercial capillary anion‐exchange column (id = 0.4 mm, L = 15 cm) using a five‐anion standard mixture. The achieved results were compared to the performance of a standard bore ion‐exchange system (id = 4 mm, L = 15 cm), which was considered as a reference. The first‐generation capillary columns exhibited a minimal reduced plate‐height value below two witnessing a good packing quality and system performance. However, compared to the standard bore system the capillary system displayed an increased apparent C ‐term which could be due to a difference in packing morphology and/or possible external band‐broadening contributions. For fast separations, the standard bore system outperformed the capillary system, while for complex separations both systems performed nearly equally well. In addition, the retention characteristics of the capillary system were investigated. To illustrate the suitability of the capillary system, the analysis of real‐world water samples originating from two local B elgian rivers was demonstrated.