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Matrix effects in the determination of β ‐receptor agonists in animal‐derived foodstuffs by ultra‐performance liquid chromatography tandem mass spectrometry with immunoaffinity solid‐phase extraction
Author(s) -
Wang Guomin,
Zhao Jian,
Peng Tao,
Chen Dongdong,
Xi Cunxian,
Wang Xiong,
Zhang Jinzhong
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200661
Subject(s) - chromatography , chemistry , clenbuterol , solid phase extraction , extraction (chemistry) , matrix (chemical analysis) , detection limit , tandem mass spectrometry , ractopamine , affinity chromatography , mass spectrometry , terbutaline , liquid chromatography–mass spectrometry , selected reaction monitoring , enzyme , biochemistry , medicine , asthma
Matrix effects in determination of three β ‐receptor agonists including salbutamol (SAL), clenbuterol, and terbutaline in animal‐derived foodstuffs were studied by ultra‐performance LC‐MS/MS with cleanup of immunoaffinity SPE column (IAC). Some animal tissue samples including pig liver, swine muscle, and fish muscle were hydrolyzed by the mixed enzyme solution or HCl solution, and the cleanup efficiencies with SAL IAC, MCX SPE column, and C 18 ‐SCX tandem columns were examined and compared by using spiked experiments. The results showed that the matrix effects in the determination of SAL and terbutaline can be eliminated with SAL IAC cleanup, and the average recoveries of SAL were 77.4∼81.5%, 79.0∼80.3%, and 85.0∼87.2% in pig liver, swine muscle, and fish muscle, respectively. The decision limit (cc α ) and detection capability (cc β ) for SAL in pig liver were 0.02 and 0.05 μg/kg, respectively.