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Parameters optimization of supercritical fluid‐ CO 2 extracts of frankincense using response surface methodology and its pharmacodynamics effects
Author(s) -
Zhou Jing,
Ma Xingmiao,
Qiu BiHan,
Chen Junxia,
Bian Lin,
Pan Linmei
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200647
Subject(s) - supercritical fluid , chromatography , response surface methodology , supercritical fluid extraction , supercritical carbon dioxide , extraction (chemistry) , chemistry , box–behnken design , steam distillation , distillation , organic chemistry
The volatile oil parts of frankincense ( B oswellia carterii B irdw.) were extracted with supercritical carbon dioxide under constant pressure (15, 20, or 25 MPa) and fixed temperature (40, 50, or 60°C), given time (60, 90, or 120 min) aiming at the acquisition of enriched fractions containing octyl acetate, compounds of pharmaceutical interest. A mathematical model was created by B ox‐ B ehnken design, a popular template for response surface methodology, for the extraction process. The response value was characterized by synthetical score, which comprised yields accounting for 20% and content of octyl acetate for 80%. The content of octyl acetate was determined by GC . The supercritical fluid extraction showed higher selectivity than conventional steam distillation. Supercritical fluid‐ CO 2 for extracting frankincense under optimum condition was of great validity, which was also successfully verified by the pharmacological experiments.