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Determination of chloramphenicol in aquatic products by graphene‐based SPE coupled with HPLC ‐ MS / MS
Author(s) -
Wu Jianbing,
Chen Linyao,
Mao Peipei,
Lu Yanbin,
Wang Huizhong
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200617
Subject(s) - chromatography , high performance liquid chromatography , chemistry , chloramphenicol , solid phase extraction , antibiotics , biochemistry
An effective analytical protocol using graphene‐based SPE coupled with HPLC ‐ MS / MS for determination of chloramphenicol ( CAP ) in aquatic products has been developed. In the present work, graphene was evaluated as SPE sorbents for the analytes enrichment and clean up. The target analytes were quantified by a triple‐quadrupole linear ion trap MS in multiple‐reaction monitoring mode. In addition, the proposed method was validated according to C ommission D ecision 2002/657/EC. The calibration curve was linear over the range of 0.5–100 ng/mL. The mean values of RSD of intra‐ and interday ranging from 1.48 to 4.29% and from 3.25 to 7.42% were obtained, respectively. In the three fortified levels, the recoveries of CAP ranging from 92.3 to 103.4% with RSD s ≤ 5.58% were obtained. The proposed method has been successfully applied to the analysis of CAP in several aquatic product samples, indicating that graphene was a potential SPE sorbent for the enrichment of trace residues in food samples.