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Molecular imprinted polymer for solid‐phase extraction of flavonol aglycones from M oringa oleifera extracts
Author(s) -
Pakade Vusumzi,
Cukrowska Ewa,
Lindahl Sofia,
Turner Charlotta,
Chimuka Luke
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200576
Subject(s) - kaempferol , myricetin , chemistry , quercetin , chromatography , flavonols , solid phase extraction , moringa , extraction (chemistry) , molecularly imprinted polymer , high performance liquid chromatography , organic chemistry , food science , antioxidant , catalysis , selectivity
Molecular imprinted polymer produced using quercetin as the imprinting compound was applied for the extraction of flavonol aglycones (quercetin and kaempferol) from M oringa oleifera methanolic extracts obtained using heated reflux extraction method. Identification and quantification of these flavonols in the Moringa extracts was achieved using high performance liquid chromatography with ultra violet detection. Breakthrough volume and retention capacity of molecular imprinted polymer SPE was investigated using a mixture of myricetin, quercetin and kaempferol. The calculated theoretical number of plates was found to be 14, 50 and 8 for myricetin, quercetin and kaempferol, respectively. Calculated adsorption capacities were 2.0, 3.4 and 3.7 μmol/g for myricetin, quercetin and kaempferol, respectively. No myricetin was observed in Moringa methanol extracts. Recoveries of quercetin and kaempferol from Moringa methanol extracts of leaves and flowers ranged from 77 to 85% and 75 to 86%, respectively, demonstrating the feasibility of using the developed molecularly imprinted SPE method for quantitative clean‐up of both of these flavonoids. Using heated reflux extraction combined with molecularly imprinted SPE , quercetin concentrations of 975 ± 58 and 845 ± 32 mg/kg were determined in M oringa leaves and flowers, respectively. However, the concentrations of kaempferol found in leaves and flowers were 2100 ± 176 and 2802 ± 157 mg/kg, respectively.