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Ultrasound‐assisted matrix solid phase dispersive extraction for the simultaneous analysis of β‐lactams (four penicillins and eight cephalosporins) in milk by high performance liquid chromatography with photodiode array detection
Author(s) -
Karageorgou Eftichia G.,
Samanidou Victoria F.,
Papadoyannis Ioannis N.
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200514
Subject(s) - chromatography , chemistry , detection limit , high performance liquid chromatography , flumequine , matrix (chemical analysis) , cefaclor , cefadroxil , solid phase extraction , cephalosporin , antibiotics , enrofloxacin , biochemistry , ciprofloxacin
The application of ultrasound‐assisted matrix solid phase dispersive extraction for the confirmatory analysis of 12 β‐lactam antibiotics in milk by high performance liquid chromatography with photodiode array detection has been proposed herein. Four penicillins (cloxacillin, dicloxacillin, oxacillin, and amoxicillin) and eight cephalosporins (cefaclor, cefadroxil, ceftiofur, cefuroxime, cefoperazone, cefazolin, cephalexin, and cefotaxime) are effectively extracted using a mixed sorbent of Quick Easy Cheap Effective Rugged Safe technique and OASIS HLB providing a matrix free from any endogenous interference. Examined analytes were well resolved on an Inertsil ODS ‐3 analytical column with a mobile phase of CH 3 COONH 4 (0.05 M) and acetonitrile delivered under a gradient program. 1,7‐Dimethyl‐xanthine was used as internal standard. The method was validated meeting the E uropean L egislation determining linearity, selectivity, stability, decision limit, detection capability, accuracy, precision, and ruggedness according to the Y ouden approach. Recoveries of all antibiotics rated from 85.0 to 115.7%, while RSD values were <12.7%. Finally, the method was successfully applied to milk samples purchased from local market.