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Molecular imprinting‐based micro‐stir bar sorptive extraction for specific analysis of G libenclamide in herbal dietary supplements
Author(s) -
Wu Xiaoli,
Liu Jie,
Wu Jinhua,
Wang Yang,
Xue Cheng,
Wang Ruoyu,
Hong Junli,
Zhou Xuemin
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200493
Subject(s) - molecularly imprinted polymer , desorption , molecular imprinting , chromatography , adsorption , extraction (chemistry) , solvent , methacrylic acid , high performance liquid chromatography , chemistry , selectivity , polymer , detection limit , monomer , materials science , organic chemistry , catalysis
A novel molecularly imprinted polymers ( MIP s) coated micro‐stir bar ( MSB ) for G libenclamide ( GM ) was developed. The MIP s, with GM as template molecular and methacrylic acid as functional monomer, were synthesized at the surface of the silylated MSB that was filled with magnetic core as substrate. Computational simulation was used for the optimal selection of functional monomers and porogen. The thickness of MIP s coating for MSB was about 10 μm, the adsorption and desorption time were about 40 and 20 min, respectively. The MIP s coated MSB possessed mechanical stability, high adsorption capacity, and good selectivity for GM . To achieve the optimum extraction performance, several parameters including extraction and desorption time, stirring rate, extraction and desorption solvent were investigated. A method for the determination of GM in herbal dietary supplements by MIP s coated MSB coupled with HPLC ‐ UV was established. The results exhibited good linear ranges of 10–6250 μg L −1 with the low limit of detection of GM (3.05 μg L −1 ) and the good recoveries (81.9–101.4%).