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Analysis of global components in Ganoderma using liquid chromatography system with multiple columns and detectors
Author(s) -
Qian Zhengming,
Zhao Jing,
Li Deqiang,
Hu Dejun,
Li Shaoping
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200441
Subject(s) - chromatography detector , chromatography , detector , ganoderma , chemistry , gas chromatography , column chromatography , high performance liquid chromatography , mass spectrometry , ganoderma lucidum , food science , electrical engineering , engineering
In present study, a multiple columns and detectors liquid chromatography system for analysis of global components in traditional Chinese medicines was developed. The liquid chromatography system was consist of three columns, including size exclusion chromatography column, hydrophilic interaction chromatography column, and reversed phase chromato‐graphy column, and three detectors, such as diode array detector, evaporative light scattering detector, and mass spectrometry detector, based on column switching technique. The developed multiple columns and detectors liquid chromatography system was successfully applied to the analysis of global components, including macromolecular (polysaccharides), high (nucleosides and sugars)‐, and low (triterpenes)‐polarity small molecular compounds in Ganoderma , a well‐known Chinese medicinal mushroom. As a result, one macromolecular chromatographic peak was found in two Ganoderma species, 19 components were identified in Ganoderma lucidum (two sugars, three nucleosides, and 14 triterpenes), and four components (two sugars and two nucleosides) were identified in Ganoderma sinense . The developed multiple columns and detectors liquid chromatography system was helpful to understand comprehensive chemical characters in TCMs.