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Liquid chromatographic separation and thermodynamic investigation of stereoisomers of darunavir on C hiralpak AD‐H column
Author(s) -
Rao Ramisetti Nageswara,
Kumar Kandukuri Nagesh,
Kumar Bompelli Sravan
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200410
Subject(s) - chemistry , chromatography , phase (matter) , amylose , methanol , resolution (logic) , capacity factor , theoretical plate , high performance liquid chromatography , amine gas treating , analytical chemistry (journal) , organic chemistry , artificial intelligence , starch , computer science
Liquid chromatographic separation of stereoisomers of darunavir on C hiralpak AD‐H , a column containing the stationary phase coated with amylose tris(3,5‐dimethylphenylcarbamate) as a chiral selector, was studied under normal‐phase conditions at different temperatures between 20 and 50°C. The effect of quality and quantity of different polar organic modifiers viz: methanol, ethanol, 1‐propanol, and 2‐propanol in the mobile phase as well as column temperature on retention, separation, and resolution was investigated and optimized. The optimum separation was accomplished using a mobile phase composed of n ‐hexane/ethanol/diethyl amine (80:20:0.1 v/v/v) at 40°C. Apparent thermodynamic parameters Δ H 0 and Δ S * were derived from the V an't H off plots (ln k ′ versus 1/ T ) and used to explain the strength of interactions between the stereoisomers and amylose tris(3,5‐dimethylphenylcarbamate) coated chiral stationary phase.