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Application of ultrahigh‐performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis
Author(s) -
Zhang WeiDong,
Wang Qing,
Wang Ying,
Wang XiaoJuan,
Pu JianXin,
Gu Yi,
Wang Rong
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200393
Subject(s) - chromatography , chemistry , repeatability , formic acid , mass spectrometry , analyte , lignan , high performance liquid chromatography , calibration curve , quantitative analysis (chemistry) , analytical chemistry (journal) , gradient elution , detection limit , stereochemistry
Lignans in the drug Fructus Schisandrae chinensis ( FSC ) exhibit potent biological activities such as antihepatotoxic, antiasthmatic, and antigastric ulcer. An ultrahigh‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry method has been developed to evaluate the quality of FSC through simultaneous qualitative and quantitative analysis of 15 lignans, including schizandrin A , B , and C ; schizandrol A and B ; gomisin B , C , D , E , G , H , J , and N ; tigloylgomisin H ; and angeloylgomisin H . The compounds were separated on a Z orbax E clipse P lus C 18 (2.1 × 100 mm, 1.8 μm) column with a gradient elution of acetonitrile and 0.1% formic acid. Lignans were identified through their retention times, accurate mass data, and characteristic ions by comparison with a reference substance. All calibration curves showed perfect linear regression ( r 2 > 0.99) within the test range. The limits of detection and quantitation fell in the ranges of 0.1–4 ng/mL for all the analytes with an injection of 10 μL. Good results were obtained with respect to repeatability (relative standard deviation <4.6%) and recovery (85.58–105.82%). Meanwhile, the entire sample analysis time was less than 10 min. This developed method provided a new basis for the overall assessment of the quality of FSC.