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Quantitative determination of aceclofenac and its three major metabolites in rat plasma by HPLC ‐ MS / MS
Author(s) -
Kim Eunyoung,
Ahn Byoungki,
Noh Keumhan,
Kang Wonku,
Gwak Hye sun
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200367
Subject(s) - aceclofenac , chemistry , chromatography , electrospray ionization , protein precipitation , formic acid , triple quadrupole mass spectrometer , mass spectrometry , coefficient of variation , electrospray , selected reaction monitoring , acetonitrile , elution , tandem mass spectrometry
We developed a method for the simultaneous quantification of aceclofenac and its three major metabolites in rat plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard ( IS ), aceclofenac, diclofenac, 4′‐hydroxyaceclofenac, 4′‐hydroxydiclofenac, and the IS were chromatographed on a reverse‐phase C 18 analytical column. The isocratic mobile phase of acetonitrile/0.1% formic acid (aq; 9:1 [v/v]) was eluted at 0.3 mL/min. Quantification was performed on a triple‐quadrupole mass spectrometer using electrospray ionization, and the ion transitions were monitored in selective reaction‐monitoring mode. The coefficient of variation in the assay precision was less than 8%, and the accuracy was 92–103%. This method was successfully used to measure the concentrations of aceclofenac and its three major metabolites in rat plasma following the oral administration of a single 20 mg/kg oral dose of aceclofenac.