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Simultaneous analysis of 11 main active components in C irsium setosum based on HPLC ‐ ESI ‐ MS / MS and combined with statistical methods
Author(s) -
Sun Qian,
Chang Lu,
Ren Yanping,
Cao Liang,
Sun Yingguang,
Du Yingfeng,
Shi Xiaowei,
Wang Qiao,
Zhang Lantong
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200359
Subject(s) - chromatography , chemistry , electrospray ionization , mass spectrometry , tandem mass spectrometry , electrospray , analyte , selected reaction monitoring , high performance liquid chromatography , analytical chemistry (journal)
A novel method based on high‐performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry was developed for simultaneous determination of the 11 major active components including ten flavonoids and one phenolic acid in C irsium setosum . Separation was performed on a reversed‐phase C 18 column with gradient elution of methanol and 0.1‰ acetic acid (v/v). The identification and quantification of the analytes were achieved on a hybrid quadrupole linear ion trap mass spectrometer. Multiple‐reaction monitoring scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. Full validation of the assay was carried out including linearity, precision, accuracy, stability, limits of detection and quantification. The results demonstrated that the method developed was reliable, rapid, and specific. The 25 batches of C . setosum samples from different sources were first determined using the developed method and the total contents of 11 analytes ranged from 1717.460 to 23028.258 μg/g. Among them, the content of linarin was highest, and its mean value was 7340.967 μg/g. Principal component analysis and hierarchical clustering analysis were performed to differentiate and classify the samples, which is helpful for comprehensive evaluation of the quality of C . setosum .