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Preparation of a stationary phase with quaternary ammonium embedded group for selective separation of alkaloids based on ion‐exclusion interaction
Author(s) -
Liu Qiaoxia,
Liang Tu,
Li Kuiyong,
Ke Yanxiong,
Jin Yu,
Liang Xinmiao
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200341
Subject(s) - chemistry , ammonium , elution , chromatography , phase (matter) , alkaloid , fraction (chemistry) , stationary phase , ionic bonding , ion , organic chemistry
A stationary phase (named QA C 10) with quaternary ammonium embedded between a propyl and a decyl chain was synthesized by immobilization of N , N ‐dimethyldecylamine on chloropropyl–silica surface. A set of representative neutral, basic, and acidic compounds was employed to evaluate its chromatographic properties. The results illustrated that QA C 10 was a mixed‐mode stationary phase possessing both hydrophobic and ionic characteristics. The QA C 10 stationary phase was further used for selective separation of alkaloids from C ortex phellodendri . Under acidic condition, alkaloids could be eluted in first 8 min, while other neutral and acidic fractions were retained better on QA C 10 column. Then, obtained alkaloid fraction was analyzed by LC‐MS/MS and 22 alkaloids were identified. Our study confirmed the advantages and application potential of the QA C 10 stationary phase for alkaloids separation.