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Determination of L‐trantinterol in rat plasma by using chiral liquid chromatography‐tandem mass spectrometry
Author(s) -
Jing Lijuan,
Li Kunjie,
Qin Fen,
Wang Xintao,
Pan Li,
Wang Yanjuan,
Cheng Maosheng,
Li Famei
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200332
Subject(s) - chromatography , chemistry , calibration curve , selected reaction monitoring , mass spectrometry , analytical chemistry (journal) , detection limit , tandem mass spectrometry , triple quadrupole mass spectrometer , enantiomer , acetonitrile , liquid chromatography–mass spectrometry , tandem , acetic acid , materials science , stereochemistry , composite material , biochemistry
A simple, sensitive, and rapid method for determination of L‐trantinterol in rat plasma was developed for the first time by using LC coupled to MS / MS based on chiral stationary phase. A baseline separation of the enantiomers of trantinterol was achieved on a C hirobiotic V column, using a mixture of acetonitrile–methanol–ammonia–acetic acid (80:20:0.01:0.02, v/v/v/v) as the mobile phase. The detection was performed on a triple‐quadrupole tandem mass spectrometer by multiple reaction monitoring mode via ESI . The calibration curve was linear in concentration range from 0.270 to 108 ng/mL in plasma with the lower limit of quantification of 0.270 ng/mL. The intra‐ and interday precision (relative standard deviation) values were within 10.9% and the accuracy (relative error) was from 2.6 to 9.2% at all quality control levels. The method has been successfully applied to a study of L‐trantinterol pharmacokinetics in rats.