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Chromatographic resolution and isotherm determination of ( R , S )‐mandelic acid on C hiralcel‐ OD column
Author(s) -
Mao Shimin,
Zhang Yan,
Rohani Sohrab,
Ray Ajay K.
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200322
Subject(s) - chemistry , chromatography , trifluoroacetic acid , resolution (logic) , elution , mandelic acid , phase (matter) , enantiomer , analytical chemistry (journal) , stereochemistry , organic chemistry , artificial intelligence , computer science
Resolution of racemic mandelic acid (( R , S )‐ MA ) and numerical determination of binary competitive isotherm of ( R , S )‐ MA on C hiralcel‐ OD column have been investigated in this study. The effects of the alcohol modifier and acidic additive in the mobile phase on the retention and enantioseparation of ( R , S )‐ MA were studied at first. The inverse method was then used to determine the competitive isotherm parameters of ( R , S )‐ MA by minimizing the sum of square deviations of the model predictions from the measured elution profiles. The results indicate that the mobile phase with 85% hexane/15% isopropanol/0.3% trifluoroacetic acid mixture gives the best resolution of ( R , S )‐ MA and competitive‐modified L angmuir isotherm provides the more accurate sorption mechanism of ( R , S )‐ MA on the cellulose tris(3,5‐dimethylphenylcarbamate) chiral stationary phase.