Chromatographic resolution and isotherm determination of ( R , S )‐mandelic acid on C hiralcel‐ OD column

Resolution of racemic mandelic acid (( R , S )‐ MA ) and numerical determination of binary competitive isotherm of ( R , S )‐ MA on C hiralcel‐ OD column have been investigated in this study. The effects of the alcohol modifier and acidic additive in the mobile phase on the retention and enantioseparation of ( R , S )‐ MA were studied at first. The inverse method was then used to determine the competitive isotherm parameters of ( R , S )‐ MA by minimizing the sum of square deviations of the model predictions from the measured elution profiles. The results indicate that the mobile phase with 85% hexane/15% isopropanol/0.3% trifluoroacetic acid mixture gives the best resolution of ( R , S )‐ MA and competitive‐modified L angmuir isotherm provides the more accurate sorption mechanism of ( R , S )‐ MA on the cellulose tris(3,5‐dimethylphenylcarbamate) chiral stationary phase.