Premium
Micellar electrokinetic chromatography systems for the separation of mixtures of charged and uncharged compounds
Author(s) -
Lamalle Caroline,
Servais AnneCatherine,
Fradi Ines,
Crommen Jacques,
Fillet Marianne
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200293
Subject(s) - chemistry , micellar electrokinetic chromatography , electrokinetic phenomena , micelle , electrolyte , sodium dodecyl sulfate , acetonitrile , electrophoresis , chromatography , selectivity , capillary electrophoresis , sodium , inorganic chemistry , organic chemistry , aqueous solution , electrode , catalysis
In this study, the migration behavior of charged and uncharged analytes was investigated under different conditions. Effective mobilities – electrophoretic mobilities under the influence of micelles – of cations, anions, and neutrals were measured at neutral, basic, and acidic pH (7.5, 11, and 2.2) using background electrolytes containing different sodium dodecyl sulfate ( SDS ) concentrations (0–90 mM) and acetonitrile ( ACN ) proportions (0–75%). SDS concentration and ACN proportion were found to have a tremendous effect on the effective mobilities and migration order of the model compounds. Although the SDS micelles preferably interact with neutrals and cations, hydrophobic bonds can also occur with anions. Cations, anions, and neutrals having rather different migration behaviors, it is possible to considerably enhance the selectivity of the method by adjusting properly the SDS concentration and the ACN proportion. These observations confirm the interest of using micellar electrokinetic chromatography not only for the separation of neutral substances but also to analyze charged compounds.