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Liquid–liquid/solid three‐phase high‐speed counter‐current chromatography, a new technique for separation of polyphenols from G eranium wilfordii M axim
Author(s) -
Liu Dan,
Ma Yan,
Gu Ming,
Janson JanChrister,
Wang Changhai,
Xiao Hongbin
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200283
Subject(s) - chromatography , chemistry , countercurrent chromatography , polyphenol , high performance liquid chromatography , ethyl acetate , acetic acid , extraction (chemistry) , organic chemistry , antioxidant
High‐speed counter‐current chromatography using a new liquid–liquid/solid three‐phase system was used for the separation of the polyphenols corilagin and geraniin from a crude extract of G eranium wilfordii M axim in one step. The optimized three‐phase system was composed of n ‐hexane/ethyl acetate/methanol/acetic acid/water and to which was added 10‐μm average diameter microspheres of cross‐linked 12% agarose at the ratio of 0.2:10:2:1:5 and 0.1 g/mL, respectively. The purities of geraniin and corilagin were 82 and 90%, which were determined by HPLC at 280 nm. A 14.5 and 7 mg of geraniin and corilagin were purified from 160 mg crude extract with the yields of 70 and 78%, respectively.