Premium
On‐line ionic liquid‐based dynamic microwave‐assisted extraction‐high performance liquid chromatography for the determination of lipophilic constituents in root of S alvia miltiorrhiza Bunge
Author(s) -
Gao Shiqian,
Yu Wei,
Yang Xiao,
Liu Zhonglin,
Jia Yilong,
Zhang Hanqi
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200267
Subject(s) - chromatography , ionic liquid , extraction (chemistry) , chemistry , analyte , sample preparation , high performance liquid chromatography , maceration (sewage) , analytical chemistry (journal) , materials science , biochemistry , composite material , catalysis
On‐line continuous sampling, ionic liquid‐based dynamic microwave‐assisted extraction high performance liquid chromatography has been developed and applied to the extraction of lipophilic constituents from root of S alvia miltiorrhiza Bunge. Several operating parameters were optimized by single‐factor and B ox– B ehnken design experiments. The type and concentration of ionic liquids, power of microwave irradiation, flow rate of sample suspension, amount, and particle size of sample were investigated. The limits of detection for tanshin‐one I, cryptotanshinone, and tanshinone II A are 0.014, 0.009, and 0.009 mg/g, respectively. The RSD s of interday and intraday were lower than 2.02 and 2.16%, respectively. The recoveries for target analytes were in the range of 90.7–101.8%. The homogeneity of the suspension and stability of the analytes were investigated and the results were satisfactory. The proposed method was compared with the off‐line ionic liquid‐based dynamic microwave‐assisted extraction, off‐line ethanol‐based dynamic microwave‐assisted extraction, ionic liquid‐based ultrasonic‐assisted extraction, and ionic liquid‐based maceration extraction. The results indicated that the proposed method is effective for the extraction of the active components in C hinese herbal medicine and has some advantages over the other methods.