Preparative separation of bioactive compounds from essential oil of F laveria bidentis ( L .) K untze using steam distillation extraction and one step high‐speed counter‐current chromatography

In order to utilize and control the invasive weed, bioactive compounds from essential oil of F laveria bidentis ( L .) K untze were studied. Steam distillation extraction and one step high‐speed counter‐current chromatography were applied to separate and purify the caryophyllene oxide, 7,11‐dimethyl‐3‐methylene‐1,6,10‐dodecatriene, and caryophyllene from essential oil of F laveria bidentis ( L .) K untze. The two‐phase solvent system containing n ‐hexane/acetonitrile/ethanol (5:4:3, v/v/v) was selected for the one step separation mode according to the partition coefficient values ( K ) of the target compounds and the separation factor (α). The purity of each isolated fraction after a single high‐speed counter‐current chromatography run was determined by high performance liquid chromatography. A 3.2 mg of caryophyllene oxide at a purity of 92.6%, 10.4 mg of 7,11‐dimethyl‐3‐methylene‐1,6,10‐dodecatriene at a purity of 99.1% and 5.7 mg of caryophyllene at a purity of 98.8% were obtained from 200 mg essential oil of F laveria bidentis ( L .) K untze. The chemical structures of these components were identified by GC ‐ MS , 1 H‐NMR , and 13 C‐NMR .