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Solid‐phase microextraction combined with comprehensive two‐dimensional gas chromatography for fatty acid profiling of cell wall phospholipids
Author(s) -
Bogusz Stanislau,
Hantao Leandro Wang,
Braga Soraia Cristina Gonzaga Neves,
de Matos França Valtenice de Cássia Rodrigues,
da Costa Marcelo Fernandes,
Hamer Russell David,
Ventura Dora Fix,
Augusto Fabio
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200256
Subject(s) - derivatization , chromatography , solid phase microextraction , chemistry , gas chromatography , detection limit , sample preparation , solid phase extraction , analyte , flame ionization detector , gas chromatography–mass spectrometry , high performance liquid chromatography , mass spectrometry
The combination of solid‐phase microextraction ( SPME ) with comprehensive two‐dimensional gas chromatography is evaluated here for fatty acid ( FA ) profiling of the glycerophospholipid fraction from human buccal mucosal cells. A base‐catalyzed derivatization reaction selective for polar lipids such as glycerophospholipid was adopted. SPME is compared to a miniaturized liquid–liquid extraction procedure for the isolation of FA methyl esters produced in the derivatization step. The limits of detection and limits of quantitation were calculated for each sample preparation method. Because of its lower values of limits of detection and quantitation, SPME was adopted. The extracted analytes were separated, detected, and quantified by comprehensive two‐dimensional gas chromatography with flame ionization detection ( FID ). The combination of SPME and comprehensive two‐dimensional gas chromatography with FID , using a selective derivatization reaction in the preliminary steps, proved to be a simple and fast procedure for FA profiling, and was successfully applied to the analysis of adult human buccal mucosal cells.