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Elucidation of steroid glycosides in Anemarrhena asphodeloides extract by means of comprehensive two‐dimensional reversed‐phase/polyamine chromatography with mass spectrometric detection
Author(s) -
Liu Zhirui,
Zhu Dongliang,
Qi Yunpeng,
Chen Xiaofei,
Zhu Zhenyu,
Chai Yifeng
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200236
Subject(s) - chromatography , chemistry , polyamine , high performance liquid chromatography , steroid , reversed phase chromatography , column chromatography , mass spectrometry , hydrophilic interaction chromatography , countercurrent chromatography , glycoside , analytical chemistry (journal) , organic chemistry , biochemistry , hormone
In the work presented here, a novel approach to comprehensive two‐dimensional liquid chromatography is evaluated. Reversed‐phase liquid chromatography was employed for the first‐dimension separation and polyamine chromatography was chosen for the second‐dimension separation mode. The two dimensions are highly orthogonal and the separation efficacy of the developed octadecylsilica × polyamine was tested by separating an extract from Anemarrhena asphodeloides . The steroid glycosides identified by comprehensive two‐dimensional liquid chromatography in this experiment were compared to those obtained for monodimensional liquid chromatography. The comprehensive two‐dimensional liquid chromatography system, thanks to the complementary separation selectivity and enhanced peak capacity provided by the two columns, allowed to distribute five compounds of low amounts otherwise unachievable by monodimensional liquid chromatography. In addition, four steroid isomers with similar fragmentation characteristics in MS/MS spectra, were newly separated based on their different chemical structures.