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Isolation of fucoxanthin from edible brown algae by microwave‐assisted extraction coupled with high‐speed countercurrent chromatography
Author(s) -
Xiao Xiaohua,
Si Xiaoxi,
Yuan Zhiquan,
Xu Xianfang,
Li Gongke
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200231
Subject(s) - fucoxanthin , chromatography , countercurrent chromatography , extraction (chemistry) , chemistry , high performance liquid chromatography , solvent , countercurrent exchange , ethyl acetate , chloroform , hexane , pigment , organic chemistry , biology , anatomy
A rapid and efficient method for the separation and purification of fucoxanthin from edible brown algae by microwave‐assisted extraction coupled with high‐speed countercurrent chromatography was developed. The algae were first extracted using microwave‐assisted extraction, then the dried extract was dissolved and directly introduced into the high‐speed countercurrent chromatography system with a two‐phase solvent system consisting of hexane‐ethyl acetate‐ethanol‐water (5:5:6:4, v/v/v/v). The isolation was done in less than 75 min, and a total of 0.83 mg, 1.09 mg, and 0.20 mg fucoxanthin were obtained from 25.0 g fresh L aminaria japonica A resch, 1.5 g dry U ndaria pinnatifida ( H arv) S ur, and 15.0 g dry S argassum fusiforme ( H arv) S etch, respectively. The purity of fucoxanthin determined by HPLC was over 90% and its structure was further identified by LC‐ESI‐MS and 1 H‐NMR .