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Evaluation of lipid removal from animal fats for the determination of organochlorine, pesticides, and polychlorinated biphenyl indicators by gas chromatography with electron capture detector
Author(s) -
Andrade Adriana S.,
Sacheto Doriane,
Hoff Rodrigo B.,
Pizzolato Tania M.
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200223
Subject(s) - chromatography , electron capture detector , chemistry , gas chromatography , polychlorinated biphenyl , elution , solid phase extraction , extraction (chemistry) , biphenyl , pesticide , matrix (chemical analysis) , detection limit , solvent , filtration (mathematics) , environmental chemistry , organic chemistry , statistics , mathematics , agronomy , biology
For cleanup of animal fat before GC analysis of organochlorine pesticides and polychlorinated biphenyls, freezing‐lipid filtration with solid‐phase extraction and matrix solid‐phase dispersion were evaluated to replace the official B razilian methodology that uses preparative alumina column chromatography. General drawbacks associated with this last technique, such as the use of large amounts of solvent, laborious and time‐consuming procedure could be avoided by using these alternative approaches. Experiments were carried out to study the performance by using different combinations of sorbents and elution solvents. Efficiency of alternative extraction methods in terms of fat removal and recovery capability was monitored by gravimetry, TLC , and GC with electron capture detection. Freezing‐lipid filtration with solid‐phase extraction afforded better clean up efficiency with recoveries in a range of 54.5 to 103.6% with the relative standard deviation of less than 10% for all compounds under investigation.