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Automated online pretreatment and cleanup recycle coupled with high‐performance liquid chromatography‐mass spectrometry for determination of deca‐bromodiphenyl ether in human serum
Author(s) -
Lin Xuexia,
Li HaiFang,
He Xiangwei,
Hashi Yuki,
Lin JinMing,
Wang Zhihua
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200211
Subject(s) - chromatography , chemistry , deca , ether , sample preparation , mass spectrometry , detection limit , linear range , high performance liquid chromatography , dilution , gas chromatography–mass spectrometry , organic chemistry , medicinal chemistry , physics , thermodynamics
Automated online SPE ‐ HPLC ‐ MS was established for the determination of deca‐bromodiphenyl ether in human serum. The online SPE with large volume injection was utilized to enhance the sensitivity. Online SPE with dilution line greatly decreased matrices effect, which enabled serum samples to be injected directly into pre‐column. Washing line was designed for the system to solve the serious residual phenomenon and reduce the risk of sample wastage and contamination. Under the optimized conditions, the linear of the method was in the range 0.1–10 ng/mL with the LOD of 0.026 ng/mL. The recoveries of serum samples spiked with deca‐bromodiphenyl ether at 0.5 ng/mL was in the range from 83.30 to 102.7% with RSD in interday less than 8.67%. The satisfactory results demonstrated that the method of online sample pretreatment and cleanup recycle were reliable for human serum analysis.