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Quantification of ampicillin in bovine milk by coupled‐column ultrahigh‐performance liquid chromatography‐tandem mass spectrometry
Author(s) -
Moura Franciane,
de Almeida Fernando G.,
Rebelo Lopes Bianca,
Bezerra Cass Quezia
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200205
Subject(s) - chromatography , chemistry , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , high performance liquid chromatography , bovine milk , sample preparation , analytical chemistry (journal)
This work reports the use of two‐dimensional (2 D ) liquid chromatography system coupled with a tandem mass spectrometry for the quantification of ampicillin in bovine milk. A restrict access media column ( RAM ‐ BSA C 8 , 50 × 2.1 mm, L una, 10 μm, 100 Å) was used in the first dimension in order to exclude macromolecules, while an ACQUITY UPLC BEH C 18 (50 × 2.1 mm, 1.7 μm) column was used in the second dimension. Three different channels of selected reaction monitoring ( SRM ) were used: 350 > 106 m / z , 350 > 160 m / z , and 350 > 192 m / z . The first transition was used for the quantification (higher intensity), and latter two for confirmation. The developed method is simple and requires a total analysis time of only 14 min/sample. The sample treatment involved only a centrifugation step for 20 min. The validated method has been successfully applied to monitor AMP residues in raw milk samples. To our knowledge, this is the first study to report the use of ultrahigh‐performance liquid chromatography ( UHPLC ) in 2 D configuration.