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Development of a cloud point extraction method for the determination of phenolic compounds in environmental water samples coupled with high‐performance liquid chromatography
Author(s) -
Ma Huihui,
Mu Fengtian,
Fan Shujuan,
Zhou Xiao,
Jia Qiong
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200170
Subject(s) - chromatography , chemistry , extraction (chemistry) , cloud point , high performance liquid chromatography , detection limit , dilution , pulmonary surfactant , solvent , sample preparation , chromatography detector , analytical chemistry (journal) , biochemistry , physics , organic chemistry , thermodynamics
A method based on ultrasound‐assisted cloud point extraction ( CPE ) was developed for the determination of phenolic compounds in water samples by high‐performance liquid chromatography coupled with diode array detection ( DAD ) ( HPLC – DAD ). A nonionic surfactant, T ergitol 15‐ S ‐7, was chosen as the surfactant. The parameters affecting the CPE efficiency, such as T ergitol 15‐ S ‐7 concentration, salt concentration, extraction temperature, incubation time, centrifugation time, ultrasonic time, sample p H , and dilution solvent were systematically evaluated and optimized. Under the optimum experimental conditions, the linear regression coefficients of the standard curves were greater than 0.9986 and the limits of detection were in the range of 1.7–6.0 μg/L. The method was shown to be reproducible and reliable with intraday and interday relative standard deviations lower than 4.0 and 5.8%, respectively. The recoveries for the addition of different concentrations of phenolic compounds to water samples were in the range of 81.1–109.4%.