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Rational development of a selection model for solvent gradients in single‐step separation of ginsenosides from P anax ginseng using high‐speed counter‐current chromatography
Author(s) -
Shehzad Omer,
Khan Salman,
Ha In Jin,
Park Youmie,
Kim Yeong Shik
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200135
Subject(s) - ginseng , chemistry , chromatography , countercurrent chromatography , mass spectrometry , electrospray ionization , analytical chemistry (journal) , solvent , phase (matter) , electrospray , high performance liquid chromatography , organic chemistry , medicine , alternative medicine , pathology
A new model of solvent gradients selection was rationally developed for the preparative separation of target compounds. The solvent gradients were selected based on a three‐stage screening process where stationary phase retention was ensured by introducing a new parameter termed as the phase ratio. The phase ratio was calculated after mixing the upper phase of a solvent system with the lower phase of a different solvent system (1:1, v/v). The developed model was applied to the one‐step separation of eight ginsenosides from P anax ginseng . Three gradients were selected on the basis of new model and eight ginsenosides, R b 1 , R b 2 , R c, R d, R e, R g 1 , R f, and R h 1 , were efficiently separated by high‐speed counter‐current chromatography coupled with evaporative light scattering detector. The structures of all compounds were characterized by electrospray‐ionization mass spectrometry and nuclear magnetic resonance spectroscopy.