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High‐performance liquid chromatography–time‐of‐flight mass spectrometry with adjustment of fragmentor voltages for rapid identification of alkaloids in rat plasma after oral administration of R hizoma corydalis extracts
Author(s) -
Hong Zhanying,
Zhao Liang,
Wang Xiaoling,
Le Jian,
Jia Jing,
Chai Yifeng,
Zhang Guoqing
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200126
Subject(s) - corydalis , chromatography , chemistry , tetrahydropalmatine , mass spectrometry , electrospray ionization , ammonium acetate , electrospray , tandem mass spectrometry , pharmacokinetics , high performance liquid chromatography , time of flight mass spectrometry , analytical chemistry (journal) , ionization , ion , pharmacology , medicine , alternative medicine , pathology , traditional chinese medicine , organic chemistry
A tandem high‐performance liquid chromatography and time‐of‐flight mass spectrometry method has been developed for rapid separation and identification of alkaloids in rat plasma after oral administration of R hizoma corydalis extracts. Compounds were separated on a Z orbax XDB‐C 18 column and the linear gradient elution was used with the mobile phase consisting of ammonium acetate–0.01% acetic acid and acetonitrile. Mass spectra were acquired by electrospray ionization in the positive ion mode. Fifteen components were detected in vitro , among which 12 components were rapidly identified based on the accurate‐mass capability of time‐of‐flight analyzer. Eleven prototype components were detected in dosed rat plasma ( in vivo ). Three isomers, which had the same [ M + H ] + ion at m / z 356.1856, were distinguished as glaucine, tetrahydropalmatine, and corybulbine by dynamic adjustment of fragmentor voltages. This study developed a rapid and sensitive method that was successfully utilized for screening the active ingredients of R hizoma corydalis in rat plasma, which provided helpful information for further pharmacokinetic research of active components in this medicine.