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Preparative purification of peoniflorin and albiflorin from peony rhizome using macroporous resin and medium‐pressure liquid chromatography
Author(s) -
Wang Rong,
Peng Xiaoguo,
Wang Liming,
Tan Beibei,
Liu Jiangyun,
Feng Yulin,
Yang Shilin
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200120
Subject(s) - chemistry , chromatography , adsorption , desorption , rhizome , freundlich equation , high performance liquid chromatography , organic chemistry , traditional medicine , medicine
Peoniflorin ( PF ) and albiflorin ( AF ) are two principal components of P aeonia species, which exhibit various biological activities such as improvement of blood circulation and immunoregulating function. To further utilization of waste parts of peony plants, an efficient method for preparative purification of these two ingredients from white peony rhizome was developed based on macroporous resin ( MAR ) and medium‐pressure liquid chromatography ( MPLC ). The separation characteristics of nine typical MAR s were investigated by static adsorption/desorption experiments, and LX 38 was revealed as optimal one. Further static experiments with LX 38 resin indicated that the adsorbents fitted well to the pseudo‐second‐order kinetics model and both L angmuir and F reundlich isotherm models. Based on the optimal process parameters, a large‐scale preparation was successfully applied. After one run treatment with LX 38, the contents of PF and AF were increased 15‐fold to 24.5 and 16.8% in the refined extract, respectively. Both purified compounds were obtained from refined extract by reversed‐phase MPLC at second‐stage separation. The process developed is better because of its low cost, high efficiency, and procedural simplicity making it a potential approach for large‐scale production of PF and AF for their further applications in functional foods and pharmaceuticals.