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Assessment of β‐lactams retention in hydrophilic interaction chromatography applying B ox– B ehnken D esign
Author(s) -
Jovanović Marko,
Rakić Tijana,
JančićStojanović Biljana,
Malenović Anđelija,
Ivanović Darko,
Medenica Mirjana
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200099
Subject(s) - chemistry , hydrophilic interaction chromatography , retention time , chromatography , cefuroxime , cefalexin , high performance liquid chromatography , cephalosporin , antibiotics , biochemistry
In this paper, the retention prediction models for mixture of β‐lactam antibiotics analyzed by hydrophilic interaction chromatography ( HILIC ) are presented. The aim of the study was to investigate the retention behavior of some organic acids and amphoteric compounds including cephalosporins (cefotaxime, cefalexin, cefaclor, cefuroxime, and cefuroxime axetil) and penicillins (ampicillin and amoxicillin). Retention of substances with acidic functional group in HILIC is considered to be interesting since the majority of publications in literature are related to basic compounds. In the beginning of the study, classical silica columns were chosen for the retention analysis. Then, preliminary study was done and factors with the most significant influence on the retention factors were selected. These factors with the impact on the retention factors were investigated employing B ox– B ehnken design as a tool. On the basis of the obtained results the mathematical models were created and tested using ANOVA test and finally verified. This approach enables the presentation of chromatographic retention in many ways (three‐dimensional (3‐ D ) graphs and simple two‐dimensional graphical presentations). All of these gave the possibility to predict the chromatographic retention under different conditions. Furthermore, regarding the structure of the analyzed compounds, the potential retention mechanisms in HILIC were suggested.