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Temperature effects on nano‐ LC column packing technology
Author(s) -
Leonardis Irene,
Capriotti Fabiana,
Cappiello Achille,
Famiglini Giorgio,
Palma Pierangela
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200081
Subject(s) - column (typography) , chromatography , chemistry , nano , chemical engineering , computer science , engineering , telecommunications , frame (networking)
We investigated the effect of temperature on the packing procedure of nano‐ LC columns (up to 50 cm) and on their performance. Several slurries of stationary phase were prepared using different solvent mixtures. Their stability was evaluated at several temperatures: 70°C, 50°C, and room temperature. At the higher temperature (70°C) the suspensions resulted to be stable for a longer time. For each slurry, we compared nano‐ LC columns packed with ultrasounds at 70°C and at room temperature. All the columns were tested with a standard mixture at 70°C, to reduce the solvent viscosity and the backpressure. Main chromatographic parameters such as the asymmetry factor, A s, the reduced plate high, h , pattern in a V an D eemter plot, the total porosity, t , and the permeability, k , were calculated and discussed. One of the nano‐ LC columns was used to separate a mixture of pesticides in a LC ‐ MS system with an electron ionization LC ‐ MS interface ( D irect‐ EI ). From our knowledge, this is the first study on the role of temperature in the efficiency of slurry‐packing procedure.