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Profiling eicosanoids and phospholipids using LC ‐ MS / MS : P rinciples and recent applications
Author(s) -
Ecker Josef
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200056
Subject(s) - chemistry , lipid signaling , arachidonic acid , lipidomics , biochemistry , eicosanoid , polyunsaturated fatty acid , fatty acid , enzyme
Eicosanoids are potent lipid mediators involved in numerous physiological and pathophysiological processes. Precursors are polyunsaturated fatty acids liberated from membrane phospholipids. Thus, profiling and quantification of these molecules has gained a lot of attention during last years. Eicosanoids and phospholipids are commonly profiled by LC ‐ MS / MS because this technique allows accurate quantification within acceptable run‐times. This article therefore focuses on liquid chromatography and the ESI ‐ MS/MS analysis of proinflammatory lipid mediators, particularly arachidonic acid ( C 20:4) derived eicosanoids and their precursors phospholipids. Recent analytical developments for quantification of these compounds are highlighted and analytical challenges are discussed. Furthermore, applications such as the use of these molecules as biomarkers are presented.