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Neuropeptide analysis with liquid chromatography‐capillary electrophoresis‐mass spectrometric imaging
Author(s) -
Zhang Zichuan,
Jia Chenxi,
Li Lingjun
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200051
Subject(s) - chemistry , chromatography , capillary electrophoresis , mass spectrometry , analyte , analytical chemistry (journal)
Herein we report the first attempt of coupling multidimensional separations to matrix‐assisted laser desorption/ionization (MALDI) mass spectrometric imaging detection. Complex neuropeptide mixtures extracted from crustaceans were first fractionated by reversed‐phase liquid chromatography (RPLC), and then subjected to a capillary electrophoresis‐mass spectrometric imaging platform. With a specific focus on orcokinin family neuropeptides, we demonstrated that these trace‐level analytes from complex neural tissue samples can be fully separated from chemical noise and interfering components and visualized as mass spectrometric imaging signals. A total of 19 putative orcokinins were detected, with highly efficient separations within the family being achieved for the first time. The results indicate that two‐dimensional separation coupling to mass spectrometric imaging can serve as a novel and powerful tool in proteomics and peptidomics studies.