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Simultaneous determination of eight bufadienolides in cinobufacini injection by HPLC coupled with triple quadrupole mass spectrometry
Author(s) -
Wu Xu,
Zhao Haiyu,
Wang Hongjie,
Gao Bo,
Yang Jian,
Si Nan,
Bian Baolin
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200036
Subject(s) - chromatography , repeatability , chemistry , gradient elution , mass spectrometry , high performance liquid chromatography , elution , analytical chemistry (journal)
A rapid and validated method was established for the simultaneous determination of eight active and toxic bufadienolides in cinobufacini injection using high performance liquid chromatography coupled with triple quadrupole mass spectrometry. These eight compounds were separated within 3 min on a C 18 analytical column with gradient elution. Eleven batches of cinobufacini injection were analyzed with good linear regression relationship ( r , 0.9979–0.9999), precisions ( RSD , 1.92–4.79%), repeatability ( RSD , 3.12–4.96%), stability ( RSD , 2.84–4.45%), and recovery (93.96–104.89%). By using the established method, the present study offered highly sensitive, specific, and speedy determination of eight bufadienolides, which promoted the quality control investigation of cinobufacini injection greatly.