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Quantitative analysis of biologically active ingredients of F ive S eeds C ombo by liquid chromatography–quadrupole time‐of‐flight mass spectrometry for quality control of commercial herbal product
Author(s) -
Chen MengLi,
Miao Lan,
Cao Jin,
Ip SiuPo,
Che ChunTao
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200034
Subject(s) - chromatography , hyperoside , verbascoside , chemistry , quercitrin , mass spectrometry , analyte , quadrupole time of flight , detection limit , active ingredient , kaempferol , adulterant , tandem mass spectrometry , quercetin , glycoside , biochemistry , organic chemistry , antioxidant , bioinformatics , biology
Five S eeds C ombo ( W u Z i Y an Z ong W an) is a traditional C hinese herbal formula composed of F ructus L ycii, S emen C uscutae, F ructus R ubi, S emen P lantaginis, and F ructus S chisandrae. This herbal prescription has been developed into herbal products by many pharmaceutical manufacturers for treating age‐related symptoms. The present study aims to develop an analytical method for the quality control of this herbal drug. Nine active ingredients including schisantherin A , schisandrin B , schisandrin, schisandrin A , quercitrin, betaine, verbascoside, hyperoside, and kaempferol were selected as the targeted analytes for the analysis. By using liquid chromatogram/quadrupole time‐of‐flight mass spectrometry ( MS ), the nine chemical compounds were determined simultaneously from the chromatogram. The parameters for MS were optimized by orthogonal array testing and the best condition of the MS for the determination of the nine marker compounds was found to be 175, 75, and 700 V for fragmentor, skimmer, and voltage of capillary, respectively. The method validation showed that this analytical method had high precision and sensitivity (limit of quantitation was smaller than 10 ng/m L for most of the analytes). The method was found to be able to demonstrate the quality of F ive S eeds C ombo from different manufacturers.