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Fingerprinting and simultaneous determination of alkaloids in P icrasma quassioides from different locations by high performance liquid chromatography with photodiode array detection
Author(s) -
Liao HuiJun,
Lai ZhengQuan,
Su JiYan,
Yi YuYang,
Li YuCui,
Lai XiaoPing,
Su ZiRen,
Lin ZhiXiu
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200025
Subject(s) - chromatography , ammonium acetate , chemistry , high performance liquid chromatography , mass spectrometry , carboxylic acid , electrospray ionization , acetonitrile , propanoic acid , analytical chemistry (journal) , stereochemistry , organic chemistry
A simple and sensitive method was developed and validated for profiling and simultaneous quantitation of seven alkaloids (6‐hydroxy‐β‐carboline‐1‐carboxylic acid, β‐carboline‐1‐carboxylic acid, β‐carboline‐1‐propanoic acid, 3‐methylcanthin‐5,6‐dione, 5‐hydroxy‐4‐methoxycanthin‐6‐one, 1‐methoxycarbony‐β‐carboline, and 4,5‐dimethoxycanthin‐6‐one) in P icrasma quassioide grown in different locations by high‐performance liquid chromatography with photodiode array detection. The analysis was conducted on a P henomenex G emini C 18 column at 35°C with mobile phase of 25 mM aqueous ammonium acetate (p H 4.0, adjusted by glacial acetate acid) and acetonitrile. A common fingerprint chromatograph under 254 nm consisting of 27 peaks was constructed for the evaluation of the similarities among 31 P . quassioide samples. Samples from G uangdong and G uangxi Provinces were found to be within group linkage and showed significant difference from that of J iangxi Province origin by using principal component analysis and hierarchical clustering analysis. In addition, the seven alkaloids were identified by electrospray ionization mass spectrometry and comparing with reference standards and literature data. All of them were determined simultaneously using the established HPLC method. This rapid and effective analytical method could be employed for quality assessment of P . quassioide , as well as pharmaceutical products containing this herbal material.